Analysis Report   |   PIPseeker 03.00.00

Force 140869

Key Metrics

Total Reads2,598,771,338Reads Above Min. Length2,347,757,659
Reads w. Valid Barcodes2,240,534,704Reads Mapped to Transcripts2,085,574,148
% Reads Mapped93.08% Reads in Cells69.35
Cells140,869Mean Reads Per Cell18,448
Duplication Rate3.477% Sequencing Saturation71.24
Transcripts in Cells483,849,470Median Transcripts Per Cell3,297
Genes Expressed in Cells36,524Median Genes Per Cell1,240
% Mito. Transcripts in Cells4.39

Clustering

Graph Based (23 Clusters)

Cluster Cell Count Cell Type (human-pbmc-v4-detailed) Feature Rank
PrimarySecondary1234567891011121314151617181920212223242526272829303132333435363738394041424344454647484950
124,901Naive CD4 TNaive CD8 TRPL31RPS14RPL32RPL34RPS27RPL7RPS6RPL21RPS18RPL3RPS13RPS25RPL35ARPL10RPS27ARPL11RPS15ARPLP2RPS29RPS23RPS4XRPS28EEF1GEEF1A1RPL19RPS3RPL13ARPS20RPL36RPS15RPS5RPS8RPL41RPL27ARPL9RPL14ENSG00000231873RPL18RPL4RPL30LDHBRPL10ARPL12RPS9RPL23ARPS3ARPS12RPS16GAS5RPL15
220,281CD14 Monocyte-FCN1ENSG00000280441S100A9ENSG00000278996MYO1FFTH1DUSP1LYZFOSMNDAATP6V0CPSAPAHNAKJUNBDPYDRBM47NCF1PLXDC2VSIRPFN1CTSDHLA-BTGOLN2VCANCSF3RTYMPSCO2RAB11FIP1COL19A1SAMHD1CTSZTAPBPLYNATG7CORO1ATBXAS1NMNAT2CD68MECOMFTLTSPOCYBAARHGAP26TNFAIP2NBPF14SERPINA1NCF2CYBBAOAHSRGN
314,653Effector Memory CD4 TCentral Memory CD4 TIL32B2MCD52TMSB4XTRACRPL41CRIP1RBFOX2EEF1A1RPS18LDHBRPL36RPL10RPS29RPL14RPL6GSTK1RPS7RPL23AS100A4RPS4XRPL36ARPS3RPL17RPL18ARPL13ACD3DRPS25RPS27RPL27ARPL19RPL34RPS15IL7RMYL12ARPS6RPS15ARPL15RPL3RPS26RPL11RPL35S100A10CD2HINT1RPS27ARPS19RPLP0RPL12S100A11
411,889B-CD74MS4A1HLA-DRB1HLA-DRAIGHMBANK1CD79ACD79BAFF3HLA-DPB1PAX5HLA-DQB1HLA-DQA1HLA-DPA1IGHDMEF2CFCRL1BACH2CD37RALGPS2TCL1AIGKCLINC00926HLA-DMAFCHSD2BIRC3CD22NIBAN3ADKZCCHC7MARCHF1VPREB3RPS11CXCR4HLA-DQA2FCRL5ADAM28STRBPLIX1-AS1EBF1SEL1L3FCER2TPD52OSBPL10HLA-DMBRPL8SIPA1L3SWAP70SNX2BCL11A
510,669Effector Memory CD8 TCytotoxic CD4 TCCL5B2MNKG7GZMAIL32CST7HCSTGZMHCD3DS100A4HLA-AMYL12AKLRK1LINC01871KLRD1TMSB4XFGFBP2UBBCD99IFITM1CD52CALM1SH3BGRL3KLRG1CD3GTRGC1HLA-EIL2RGGZMBCTSWLYARMYL12BTRACID2CD3ECD2ANXA1FCRL6CCL4PRF1PLEKTRGC2LITAFIFITM2RPL36ALSAMD3S100A6HLA-FPYHIN1UBC
69,715Natural Killer-GNLYNKG7KLRD1KLRF1GZMACST7TYROBPPRF1CTSWIFITM1IFITM2GZMBKLRB1HCSTFCER1GIL2RBUBBKLRK1FGFBP2B2MTRDCPLAC8FCGR3ACD247SH2D1BHLA-ACD63HOPXCCL5CCL4CX3CR1CALM1MYL12ARAC2ITGB2PLEKID2SRGNSAMD3GZMHLITAFMYOM2PYHIN1SERF2ARL4CTTC38SPON2DBILINC00299APMAP
77,963Naive CD4 TNaive CD8 TTPT1LEF1RPLP2TCF7RPS14RPL31RPL32RPS12RPS3ARPS6RPS25RPS13RPS8RPS28RPL34RPL9RPL35ARPL7RPL30RPS29RPS15ARPL11RPL21RPS27ARPS20GAS5RPS23RPL36MAML2LDHBRPL37RPS16RPS5RPS21EEF1GRPL5RPS17RPL4RPL19TXKBCL11BPABPC1EEF1A1RPL18RPL36ARPS3RPS18RPL10ARPS4XRPS9
87,046Central Memory CD8 TCD4 TPRKCHSKAP1SYNE2ARHGAP15KLF12RORACD247BCL11BIL32ETS1TPT1MBNL1TC2NSLFN12LCDC42SE2IL7RTTC39CCDC14AANK3TNIKFYNPHACTR2PIP4K2ASPOCK2THEMISPARP8CD96PCNX1PPP2R5CCCND3ARL4CINPP4BPTPRCAKT3ATXN1EPB41VIMRIPOR2RPS2ANKRD44MT-ND1FNBP1AAK1HLA-AMYH9SORL1SMCHD1ZBTB20LRBAHLA-C
96,477High Mito-MALAT1MT-CO2MT-ND4MT-ATP6MT-CYBMT-CO3MT-CO1MT-ND3MT-ND2MT-ND1ARHGAP15RIPOR2CD247SKAP1PRKCHBCL11BCCND3MBNL1MT-ND5FOXP1ARHGEF18ANKRD44MAML2TC2NKLF12SERINC5TNRC6BPACS1ZBTB20BCL2PITPNC1PRKCAINPP4BDOCK10PCED1BCAMK4TXKAAK1LEF1CDC42SE2CELF2EPB41TCF7ANK3CD96N4BP2L2TMC8PDE7ALUC7L3ITK
105,118CD4 T-LEF1MAML2TCF7BCL11BTPT1FOXP1PRKCACCND3SERINC5ARHGAP15CAMK4SKAP1RPS2PRKCHCD247TXKPDE3BPDE7ABACH2MBNL1PACS1RIPOR2TRABD2APITPNC1ATMZBTB20ETS1DOCK10ANKRD44THEMISKLF12BCL2EPB41AAK1LTBINPP4BITKTNRC6BNDFIP1ENSG00000235979PCED1BNOP53CDC42SE2NELL2ABLIM1ARHGEF18TMC8TC2NRPL13RAPGEF6
114,916CD16 MonocyteCD14 MonocyteTCF7L2COTL1LYNLST1NAP1L1AIF1PSAPIFI30FCGR3AMTSS1SAT1SERPINA1FTLCTSSTNFRSF1BPECAM1CST3NEAT1LYSTFTH1HCKPOU2F2FCER1GUTRNCDKN1CLILRB1ACTBGNAI2RNF149ZEB2TBXAS1TYROBPRAP1GAP2IFITM3LILRB2WARS1CD68CSF1RCYBBFGL2CPPED1CEBPBITGALLRRFIP1PTPRJSSH2MED13LPELATONGRK3FGR
124,215Naive CD8 TNaive CD4 TCD8BRPS6RPS18RPL31RPL32RPS5RPL7RPS14RPL21RPL3RPL34RPS28RPS13LINC02446RPS8RPS25RPL19RPS12RPL12RPS23RPL10ARPS4XRPS19EEF1GRPS3EEF1A1RPL10RPS9RPL23ARPS15RPL15RPL14RPL13ARPS3ARPLP2RPL4RPL27ARPS27ARPS15ARPL35ARPL36ARPL11RPL18RPS29KLRK1GAS5RPL9RPS16RPL36RPS17
132,908CD14 Monocyte-ENSG00000280441FCN1ENSG00000278996S100A9MYO1FFTH1DUSP1ATP6V0CPFN1LYZAHNAKPSAPJUNBTGOLN2ENSG00000223598DPYDMNDAVSIRTAPBPPLXDC2RBM47LYNFOSCTSDNCF1TBXAS1SAMHD1NMNAT2SSH2CYBACORO1AHLA-BATG7COL19A1ARHGEF18DOCK2RAB11FIP1MECOMTYMPSTK10CTSZCD68VMP1RNF149FOXN3SERPINA1NBPF14MED13LSTK17BSCO2
142,408CD16 Monocyte-AIF1FTLLST1IFI30TYROBPS100A6FCER1GCTSSS100A4S100A11SERPINA1SAT1OAZ1FCGR3APSAPPELATONACTBIFITM3MYL6TMSB4XLGALS1ARPC3IFITM2MS4A14MS4A6ECD74MS4A7CSF1RNAP1L1TCF7L2FGL2LILRB2CALM2ANXA2HLA-DRB1HLA-DPA1CLEC12ANPC2OAS1LRRC25CDKN1CCASP1LYZPECAM1ASAH1COTL1WARS1LYNBCL2A1CD68
151,957Conventional Dendritic 2-CST3HLA-DRAHLA-DPA1HLA-DRB1HLA-DPB1LYZHLA-DQB1CD74SAMHD1ACTBHDAC9HLA-DMAHLA-DMBCCDC88ACOTL1VIMHLA-DQA1CPVLHLA-DQA2FGL2HIP1GRNAFF3FLT3KCTD12UVRAGCIITASULF2RAC1ANXA2CTSZJAMLLGALS1TMSB10FCER1AGAPDHITGAXCCSER1IFI30DPYSL2GPX1ENSG00000235979GSTP1LRMDASYKNEGR1RPS2XPO5GNAI2ETV6
161,650CD14 MonocyteHSPCCOL19A1NMNAT2PAK5GAB2SCML2OBI1-AS1GEMIN6NELL1LINC02728LINC00649RBMS1CFAP46ENSG00000237074DENND2BCCDC85ATRIM66PDE7BMIRLET7IHGENSG00000264876NR6A1IFIT2RALYLINTS2ENSG00000229755TCF23ENSG00000233005TECTACLIC5DHRS7CCOPS8-DTFRRS1LGAS7CFL1DNAI3CSMD2WWOXNDUFAF6ENSG00000289368OPCMLLRIG3-DTBAZ1BLINC01320CDC14AIMPG2LINC01036KCNA2INPP5DCR2RFX7ENSG00000242880
171,419Plasmacytoid Dendritic-TCF4IRF8FCHSD2PTPRSCUX2APPLILRA4RABGAP1LUGCGBCL11AIRF7RHEXITM2CMPEG1ENSG00000225885CCDC50JCHAINPLXNA4GZMBPDE4BLINC01374MAPKAPK2CARD11LINC01478NIBAN3SERPINF1PLAC8AFF3RUNX2CCDC88AIL3RASPIBTGFBITAGLN2FHIP1ACYB561A3GRAMD1BEPHB1CD2APBLNKRGS7PLD4CD74CD4CST3NRP1PPP1R14BCLEC4CTXNDC5P2RY14
18813B-MS4A1IGHMCD74CD79ABANK1CD79BAFF3PAX5IGHDHLA-DRB1FCRL1BACH2HLA-DQB1HLA-DQA1RALGPS2HLA-DRAHLA-DPB1TCL1AIGKCLINC00926MEF2CCD22HLA-DPA1NIBAN3BIRC3FCHSD2ADKEBF1FCRL5CD37ZCCHC7MARCHF1STRBPHLA-DMAOSBPL10FCER2SEL1L3LIX1-AS1CXCR4IL4RTPD52CAMK2DBCL11ASIPA1L3VPREB3GNG7ADAM28BLKHLA-DQA2IKZF3
19736Platelet-PF4PPBPGNG11CAVIN2ITGA2BTUBB1SPARCGPX1NRGNTAGLN2CLUF13A1OAZ1NCOA4ITGB3CTSAOST4HLA-ETUBA4ASH3BGRL3CCL5TLN1MPIG6BPF4V1TMSB4XTREML1H2AC6H3-3APRKAR2BACRBPMYL6RGS10VCLENSG00000236449LIMS1CMTM5MYL12AENSG00000236304TIMP1MYL9PTGS1RGS18ENSG00000288882SAT1CALM3GRAP2NAP1L1ACTBMAXPKM
20367Naive CD8 TNaive CD4 TRPS29EEF1A1RPS6RPL32RPS18RPL41RPL34RPL21RPL31RPS27RPS25RPS8RPS27ARPS14RPL23ARPS3RPS28RPL10RPS15ARPL35ARPS4XRPL7RPLP2RPS12RPS20RBFOX2RPL19RPL9RPL36ARPL37RPL36RPS13RPS3ARPL27ARPS23RPL12RPL27RPL13ARPL30RPL26RPL3RPL39RPL35RPS16RPS7TPT1RPS17RPS19RPL18ARPL11
21359Plasma-JCHAINMZB1TXNDC5HSP90B1PPIBIGHA1TNFRSF17SUB1UBE2J1SEC11CIGLC1MAN1A1PDIA4HSPA5DERL3IGHA2TENT5CPOU2AF1CPNE5XBP1SPCS2ISG20LMAN1MYO1DFKBP11IGKCIFNG-AS1ENSG00000287092EAF2SEC61BHSH2DGNG7PDIA6TXNDC11SSR4CD79ASSR3CD38SPATS2EIF2AK3RHEXITM2CHERPUD1ZBP1SEL1L3MEF2CCD59SPCS3GLCCI1RPN2
22210HSPC-SOX4RPS24ZFAS1SNHG29STMN1GIHCGCDK6ENSG00000258344HNRNPA1RNF220SMIM24SERPINB1TSC22D1SPINK2AIF1RACK1RPLP0FAM30ATALDO1CATCD82IMPDH2GAPDHEEF2TXNHTR1FSNHG6BEX3GSTP1NPM1CD34HSP90AB1RPS11MSI2CASC15APEX1GAS5TKTENSG00000289364HMGA1NREPMYBANKRD28ST8SIA6CYTL1NFE2PRSS57ETV6ATP8B4RPL7A
23199CD14 MonocyteHSPCNEAT1AOAHDPYDARHGAP26PLXDC2VCANATG7LYNZEB2LRMDAMYO1FPLCB1RBM47FCN1RAB11FIP1LRRFIP1DOCK8CSF3RLYZVMP1SLC8A1GAB2SSH2TBXAS1ETV6DOCK2ZSWIM6FOXN3MED13LCUX1LYSTMCL1IRAK3DENND1APSAPCHN2SULF2USP3GNAQJARID2MAML3SRGNSPIDRPICALMMIR23AHGNUMBPRKCBJMJD1CMYO9BZFAND3